Skip to main content
. 2023 Jan 20;5(1):fcad010. doi: 10.1093/braincomms/fcad010

Figure 9.

Figure 9

The level of full-length HTT does not change in zQ175 brains from 1 to 6 months of age. (A) The level of endogenous mouse HTT, as detected by the MAB2166-CHDI-1414, was higher in wild-type mice that have two copies of mouse HTT compared to heterozygous zQ175 mice that have one copy. The complete HTRF assay data set for all nine CNS regions is shown in Supplementary Fig. 11. There was no change in the levels of endogenous mouse HTT in zQ175 mice. N = 6/genotype/age. Error bars are mean ± SEM. The test statistic, degrees of freedom and P-values for the two-way ANOVA are provided in Supplementary Table 9. The one statistical difference is shown ***P ≤ 0.001. WT = wild type, AU = arbitrary units. (B) Total full-length HTT (mutant and wild-type) was detected using the D7F7-MAB5490 assay. The complete HTRF assay data set for all nine CNS regions is shown in Supplementary Fig. 12. The levels of total full-length HTT were comparable between wild-type and zQ175 mice with no evidence that the mutation resulted in a reduction in full-length HTT levels. N = 6/genotype/age. Error bars are mean ± SEM. The test statistic, degrees of freedom and P-values for the two-way ANOVA are provided in Supplementary Table 10. Statistical differences indicate when there was a difference from 1 month to the next, and the age at which changes were first detected **P ≤ 0.01, ***P ≤ 0.001. WT = wild type, AU = arbitrary units. (C) Western blot of cortical lysates from zQ175 mice at 2 and 6 months of age probed with MW1 to detect mutant full-length HTT. HDAC4 was used as a loading control (see Supplementary Fig. 13 for full-length blot). Size standards are in kDa. Quantification howed that the levels of mutant HTT were comparable between 2- and 6-month-old zQ175 cortices. N = 4. WT = wild type.