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Activity-temperature profiles of wild-type subtilisin E (○), subtilisin E variant 5-3H5 (●), and thermitase (◊). Variant 5-3H5 was obtained by directed evolution. It is the subtilisin E eightfold mutant derivative Pro14Leu-Asn76Asp-Asn118Ser-Ser161Cys-Gly166Arg-Asn181Asp-Ser194Pro-Asn218Ser. Reprinted from reference 374 with permission of the publisher.
