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. 2023 Jan 25;17(1):e0010802. doi: 10.1371/journal.pntd.0010802

Fig 3. Genetic relationships among the Plasmodium parasites analyzed by RNA-seq.

Fig 3

(A) Neighbor-joining tree showing the relationships among cytochrome B sequences from the P. ovale parasites studied in this study (red) and sequences from NCBI (blue). Note that the sequences from the A2, B1 and B2 infections cluster with P. ovale curtisi sequences while the sequence from infection C2 clusters with P. ovale wallikeri sequences. The number next to each branch indicates the percentage of replicate trees in which the sequences clustered together based on 500 bootstraps. (B) Proportion of pairwise nucleotide differences between pairs of P. falciparum (top) and P. ovale (bottom) infections based on positions covered at >20X. Note the higher proportion of pairwise nucleotide differences in pairs including the C2 infection, consistent with its P. ovale wallikeri determination.