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. 2023 Jan 25;19(1):e1011118. doi: 10.1371/journal.ppat.1011118

Fig 3. Cellular localization and protein partner interactions of PfCYP19B point to its ER-stress-related function.

Fig 3

A) Live cell imaging of P. falciparum 3D7 parasite line with endogenously tagged PfCYP19B (green) counterstained with Hoechst33342 (blue). (B) Immunofluorescence microscopy of cells overexpressing PfCYP19B (red) with the ER marker PfBiP (green), counterstained with DAPI. Pearson’s colocalization coefficient for PfCYP19B and PfBiP in each image is shown on the right. (C) Western blot showing successful pull-down of PfBiP using episomally expressed HA-tagged PfCYP19B. An empty Vector control line was used as a negative control.