Table 1. Top mass-spectrometry hits from PfCYP19B pull-down experiment.
| Accession | Description | Symbol | Coverage [%] | # Peptides | # PSMs | Function | Ratio CYP19B/EVC | present in # of replicates |
|---|---|---|---|---|---|---|---|---|
| Rings | ||||||||
| PF3D7_0917900 * | heat shock protein 70 | BIP | 44 | 26 | 95 | protein folding, stress response | 26 | 3 |
| PF3D7_1115600 * | peptidyl-prolyl cis-trans isomerase | CYP19B | 56 | 9 | 39 | protein folding, stress response, | 200 | 3 |
| PF3D7_0821700 * | 60S ribosomal protein L22 | - | 7 | 1 | 10 | ribosome | 6 | 3 |
| PF3D7_1402500 * | ribosomal protein S27a | - | 15 | 2 | 8 | ribosome | 7 | 3 |
| PF3D7_1200600 * | shewanella-like protein phosphatase 2 | SHLP2 | 18 | 5 | 7 | cell invasion, apical complex | 4 | 3 |
| PF3D7_1026000 | conserved Plasmodium protein | - | 4 | 1 | 3 | unknown | 3 | 2 |
| PF3D7_0831400 | Plasmodium exported protein | - | 4 | 1 | 2 | exported | 6 | 3 |
| PF3D7_0729400 | ribosome biogenesis protein BRX1 | - | 5 | 2 | 2 | ribosome maturation | 4 | 3 |
| PF3D7_0827900 | protein disulfide-isomerase | PDI8 | 2 | 1 | 2 | protein folding, stress response | 3 | 3 |
| PF3D7_1368400 | ribosomal protein L1 | - | 9 | 2 | 2 | ribosome | 3 | 3 |
| PF3D7_0102500 | erythrocyte binding antigen-181 | EBA181 | 1 | 1 | 1 | exported, cell adhesion, micronemes | 5 | 1 |
| PF3D7_0928500 | conserved Plasmodium protein | - | 13 | 1 | 1 | unknown | 4 | 2 |
| PF3D7_1207100 | pre-rRNA-processing protein ESF1 | - | 1 | 1 | 1 | ribosome maturation | 4 | 3 |
| PF3D7_0503000 | 50S ribosomal protein L28 | - | 6 | 1 | 1 | ribosome, apicoplast | 4 | 3 |
| PF3D7_0510500 | topoisomerase I | TopoI | 1 | 1 | 1 | DNA metabolism | 3 | 3 |
| PF3D7_1136400 | signal recognition particle subunit SRP72 | SRP72 | 1 | 1 | 1 | transport | 3 | 3 |
| Trophozoites | ||||||||
| PF3D7_0917900 * | heat shock protein 70 | BIP | 46 | 26 | 84 | protein folding, stress response | 30 | 3 |
| PF3D7_1115600 * | peptidyl-prolyl cis-trans isomerase | CYP19B | 56 | 9 | 34 | protein folding, stress response, | 200 | 3 |
| PF3D7_1110400 * | RNA-binding protein | - | 2 | 3 | 6 | unknown | 4 | 3 |
| PF3D7_0814000 * | 60S ribosomal protein L13-2 | - | 20 | 4 | 4 | ribosome | 4 | 3 |
| PF3D7_1352500 | thioredoxin-related protein | - | 9 | 2 | 3 | stress response | 200 | 2 |
| PF3D7_1020000 | RNA-binding protein 34 | RBM34 | 4 | 1 | 3 | unknown | 5 | 3 |
| PF3D7_0210100 | 60S ribosomal protein L37ae | - | 26 | 2 | 3 | ribosome | 5 | 3 |
| PF3D7_0827900 | protein disulfide-isomerase | PDI8 | 4 | 2 | 3 | protein folding, stress response | 4 | 3 |
| PF3D7_0614500 | 60S ribosomal protein L19 | RPL19 | 10 | 2 | 2 | ribosome | 5 | 3 |
| PF3D7_0928500 | conserved Plasmodium protein | - | 13 | 1 | 2 | unknown | 4 | 3 |
| PF3D7_1222300 | endoplasmin | GRP94 | 1 | 1 | 1 | folding, export | 200 | 2 |
| PF3D7_1207100 | pre-rRNA-processing protein ESF1 | - | 3 | 1 | 1 | ribosome maturation | 200 | 3 |
| PF3D7_1303800 | conserved Plasmodium protein | - | 0 | 1 | 1 | unknown | 5 | 2 |
| PF3D7_0723900 | RNA-binding protein | - | 1 | 1 | 1 | unknown | 3 | 3 |
| Schizonts | ||||||||
| PF3D7_0917900 * | heat shock protein 70 | BIP | 42 | 23 | 68 | protein folding, stress response | 23 | 3 |
| PF3D7_1115600 * | peptidyl-prolyl cis-trans isomerase | CYP19B | 43 | 8 | 31 | protein folding, stress response | 200 | 3 |
| PF3D7_0614500 | 60S ribosomal protein L19 | RPL19 | 5 | 1 | 2 | ribosome | 200 | 2 |
| PF3D7_1033300 | conserved protein | - | 8 | 1 | 1 | unknown | 3 | 2 |
Number of detected peptides and peptide-spectrum matches (PSM) are shown. Column “Ratio CYP19B/EVC” indicates the ratio between signal abundance from pull-down performed on Pfcyp19b-OE parasite line over signal abundance in the empty Vector control (EVC, background). Only samples with a ratio ≥ 3 are shown in this table; ratios of 200 indicate that there was no peptide signal detected in the control background sample (see Materials and Methods). Pull-downs were performed independently in three biological replicates per each distinct IDC stage and mean values are shown.
*—High-confidence hits (present in all 3 out of 3 replicates
#PSM > 3, Pfcyp19b-OE/EV ratio ≥ 3).