Fig 2. Example data set with different cytoskeletal fiber types.
A: Exemplary pictures of the data set. For every stiffness (1,5,10,30 kPa PA gel, and glass) 30 images of 5 channels (phase, tubulin, vimentin, actin, DAPI) are available. B: Comparison of filament length found for microtubuli, vimentin, and actin using FS2.0 with settings optimized. C: Comparison of filament curvature found for microtubuli, vimentin, and actin using FS2.0 with settings optimized. We performed a Kolmogorov–Smirnov test between each pair of conditions. n.s. = >0.05, * = <0.05, ** = <0.01, all other conditions <0.001.