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. 2023 Feb 6;18(2):e0279336. doi: 10.1371/journal.pone.0279336

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© 2023 Hauke et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A: Raw image of hMSC on collagen-I coated glass. B: Result of FS2.0 filtering during pre-processing. C: Filaments found using straight filament detection. D: Filaments found using curved filament detection. E: Pixel-wise comparison of filaments found with straight and curved settings. Straight settings used as ground truth. Orange: Pixel only in straight detection. Red: Pixel only in curved detection. Blue: Pixel present in both settings. F: Object-wise comparison of filaments found with straight and curved settings. Straight settings used as ground truth. Orange: Object only in straight detection. Red: Object only in curved detection. Blue: Object present in both settings. Comparison was done with setting so that 75% of the object needs to be matched to count as same object. G: Histogram of object size for all three filament categories, colors matched to pictures. H: Comparison of all filaments in the ground truth data set for straight and curved settings. We performed a Kolmogorov–Smirnov test between each pair of conditions. All p-values are <0.001.