Figure 2. Analytical sensitivity of one-tube nested real-time PCR. (A) DNA extracted from C. baileyi purified oocysts; (B) DNA extracted from fecal samples added with C. baileyi oocysts. 105 oocysts (light green), 104 oocysts (blue), 103 oocysts (black), 102 oocysts (orange), 101 oocysts (yellow), and 100 oocysts (dark green).
