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. 2022 Mar 21;31(1):e000522. doi: 10.1590/S1984-29612022017

Table 1. Primers used in one-tube nested real-time PCR targeting the 18S rRNA gene of Cryptosporidium spp.

Primers Position * 5´- 3´ sequences Amplified product (bp) Reference
NRT18SF 584-616 GTTGTTGCAGTTAAAAAGCTCGTAGTTGGATT ~457 This study
NRT18SR 1010-1040 ACTTTGATTTCTCATAAGGTGCTGAAGGAGT
CPB-DIAGF 599-619 AAGCTCGTAGTTGGATTTCTG ~428 Johnson et al. (1995)
CPB-DIAGR 1006-1026 TAAGGTGCTGAAGGAGTAAGG
*

Position on C. baileyi 18S rRNA gene (GenBank accession number AF093495).