Table 1. Primers used in one-tube nested real-time PCR targeting the 18S rRNA gene of Cryptosporidium spp.
Primers | Position * | 5´- 3´ sequences | Amplified product (bp) | Reference |
---|---|---|---|---|
NRT18SF | 584-616 | GTTGTTGCAGTTAAAAAGCTCGTAGTTGGATT | ~457 | This study |
NRT18SR | 1010-1040 | ACTTTGATTTCTCATAAGGTGCTGAAGGAGT | ||
CPB-DIAGF | 599-619 | AAGCTCGTAGTTGGATTTCTG | ~428 | Johnson et al. (1995) |
CPB-DIAGR | 1006-1026 | TAAGGTGCTGAAGGAGTAAGG |
Position on C. baileyi 18S rRNA gene (GenBank accession number AF093495).