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. Author manuscript; available in PMC: 2023 Feb 6.
Published in final edited form as: Anal Chem. 2021 Feb 2;93(6):3196–3201. doi: 10.1021/acs.analchem.0c04629

Figure 5.

Figure 5.

fNRBC isolation from maternal PBMCs. (a) fNRBCs from cord blood were labeled with PE-anti-CD71 antibody and sorted by S-eDAR. Enriched fNRBCs were identified by immunostaining with APC-anti-HbF and Alexa Fluor 488 anti-CD45 antibodies and with DAPI. fNRBCs were DAPI+, CD71+, HbF+, and CD45−, and white blood cells were DAPI+, CD71−, HbF−, and CD45+. One fNRBC is circled in white. In FISH analysis, male fNRBCs exhibited one red dot (arrow) indicating the SRY gene on the Y chromosome and one cyan dot (arrow) indicating the X chromosome. (b) Mononuclear cells from cord blood were mixed with PBMCs from healthy adult female donors. fNRBCs were enriched by S-eDAR and identified by immunostaining and FISH analysis. Scale bars, 10 μm.