| Antibody |
Anti-Serotonin (rabbit polyclonal) |
MilliporeSigma |
Cat #: S5545, RRID:AB_477522
|
(1:1000) |
| Antibody |
Anti-Sv2 (mouse monoclonal) |
DSHB; (Buckley and Kelly, 1985) |
Cat #: SV2, RRID:AB_2315387
|
(1:50) |
| Antibody |
Anti-Sox2 (rabbit polyclonal) |
GeneTex |
Cat #: GTX124477, RRID:AB_11178063
|
(1:500) |
| Antibody |
Anti-Fluorescein Polyclonal Antibody, POD Conjugated (sheep polyclonal) |
Roche |
Cat #: 11426346910, RRID:AB_840257
|
(1:2000) |
| Antibody |
Anti-GFP (rabbit polyclonal) |
Thermo Fisher Scientific |
Cat #: A11122, RRID:AB_221569
|
(1:1000) |
| Antibody |
zn-12 (mouse monoclonal) |
Zebrafish International Resource Center |
Cat #: zn-12, RRID:AB_10013761
|
(1:200) |
| Antibody |
Anti-Rabbit Alexa Fluor 647 (goat polyclonal) |
Thermo Fisher Scientific |
Cat #: A32733, RRID:AB_2633282
|
(1:500) |
| Antibody |
Anti-Mouse Alexa Fluor 647 (goat polyclonal) |
Thermo Fisher Scientific |
Cat #: A32728, RRID:AB_2633277
|
(1:500) |
| Antibody |
Anti-Rabbit Alexa Fluor 568 (goat polyclonal) |
Thermo Fisher Scientific |
Cat #: A-11036, RRID:AB_10563566
|
(1:500) |
| Antibody |
Anti-Mouse Alexa Fluor 568 (goat polyclonal) |
Thermo Fisher Scientific |
Cat #: A-11031, RRID:AB_144696
|
(1:500) |
| Antibody |
Anti-Rabbit Alexa Fluor 488 (goat polyclonal) |
Thermo Fisher Scientific |
Cat #: A32731, RRID:AB_2633280
|
(1:500) |
| Commercial assay and kit |
TSA Plus Cyanine 5 |
Akoya Biosciences |
Cat #: NEL705A001KT |
(1:50) |
| Sequence-based reagent |
piezo2 in situ probe (originally referred to as piezo2b) |
Faucherre et al., 2013
|
N/A |
|
| Sequence-based reagent |
atoh1a gRNA, 5’-GGA GAC TGA ATA AAG TTA TG-3’ |
Pickett et al., 2018
|
N/A |
|
| Sequence-based reagent |
Mbait gRNA, 5’-GGC TGC TGC GGT TCC AGA GGT GG-3’ |
Kimura et al., 2014
|
N/A |
|
| Sequence-based reagent |
Zebrafish piezo2 HCR v3.0 probe |
Molecular Instruments |
N/A |
Used at 2 pmol |
| Chemical compound and drug |
MS-222 |
MilliporeSigma |
Cat #: E10521
|
|
| Chemical compound and drug |
(Z)–4-Hydroxytamoxifen (4-OHT) |
MilliporeSigma |
Cat #: H7904 |
Used at 10 μM |
| Strain and strain background (Danio rerio) |
AB (Wild-Type) |
Zebrafish International Resource Center |
ZIRC Cat# ZL1, RRID:ZIRC_ZL1
|
|
| Genetic reagent (Danio rerio) |
Tg(actb2:LOXP-BFP-LOXP-DsRed)
|
Kobayashi et al., 2014
|
Tg(actb2:LOXP-BFP-LOXP-DsRed)sd27Tg, ZFIN: ZDB-TGCONSTRCT-141111–5 |
|
| Genetic reagent (Danio rerio) |
Tg(atoh1a:nls-Eos)
|
Pickett et al., 2018
|
Tg(atoh1a:nls-Eos)w214Tg, ZFIN: ZDB-TGCONSTRCT-190701–2 |
|
| Genetic reagent (Danio rerio) |
Tg(atoh1a:lifeact-EGFP)
|
This study |
Tg(atoh1a:lifeact-EGFP)w259Tg
|
|
| Genetic reagent (Danio rerio) |
TgBAC(ΔNp63:Cre-ERT2)
|
This study |
TgBAC(ΔNp63:Cre-ERT2)w267Tg
|
|
| Genetic reagent (Danio rerio) |
Tg(sox10:Cre)
|
Kague et al., 2012
|
Tg(Mmu.Sox10-Mmu.Fos:Cre)zf384, ZFIN: ZDB-TGCONSTRCT-130614–2 |
|
| Genetic reagent (Danio rerio) |
Gt(ctnna-citrine)
|
Trinh et al., 2011
|
Gt(ctnna-citrine)ct3aGt, ZFIN: ZDB-ALT-111010–23 |
|
| Genetic reagent (Danio rerio) |
Tg(sp7:mCherry)
|
Singh et al., 2012
|
Tg(Ola.Sp7:mCherry-Eco.NfsB)pd46Tg, ZFIN: ZDB-TGCONSTRCT-120503–4 |
|
| Genetic reagent (Danio rerio) |
Tg(p2rx3a>mCherry)
|
Palanca et al., 2013
|
Tg(Tru.P2rx3a:LEXA-VP16,4xLEXOP-mCherry)la207Tg, ZFIN: ZDB-TGCONSTRCT-130307–1 |
|
| Genetic reagent (Danio rerio) |
Tg(trpa1b:EGFP)
|
Pan et al., 2012
|
TgBAC(trpa1b:EGFP)a129Tg, ZFIN: ZDB-TGCONSTRCT-120208–2 |
|
| Genetic reagent (Danio rerio) |
Tg(p2rx3b:EGFP)
|
Kucenas et al., 2006
|
Tg(p2rx3b:EGFP)sl1Tg, ZFIN: ZDB-TGCONSTRCT-070117–110 |
|
| Genetic reagent (Danio rerio) |
Tg(krt4:DsRed)
|
Rieger and Sagasti, 2011
|
Tg(krt4:DsRed)la203Tg, ZFIN: ZDB-TGCONSTRCT-120127–5 |
|
| Genetic reagent (Danio rerio) |
edadt1261
|
Harris et al., 2008
|
edadt1261, ZFIN: ZDB-ALT-090324–1 |
|
| Genetic reagent (Danio rerio) |
fgf8adhiD1Tg/+
|
Amsterdam et al., 2009
|
fgf8adhiD1Tg/+, ZFIN: ZDB-ALT-010427–4 |
|
| Software and algorithm |
FIJI |
http://fiji.sc
|
RRID:SCR_002285
|
|
| Software and algorithm |
Imaris |
Bitplane |
RRID:SCR_007370
|
|
| Other |
Fetal bovine serum |
Gibco |
Cat #: 10082–139 |
|
| Other |
Normal goat serum |
Abcam |
Cat #: ab7481, RRID:AB_2716553
|
|
| Other |
DAPI |
MilliporeSigma |
Cat #: 508741 |
Used at 5 ng/μl |
| Other |
AM1-43 |
Biotinium |
Cat #: 70024 |
Used at 15 μm |
| Other |
Alizarin Red S |
ACROS Organics |
Cat #: 400480250 |
Used at 0.01% |
| Other |
Proteinase K |
Thermo Fisher Scientific |
Cat #: 100005393 |
Used at 0.1 mg/ml |
| Other |
Hoechst 3342 |
Thermo Fisher Scientific |
Cat #: H3570 |
Used at 5 ng/μl |
