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. 2023 Jan 30;2023:9645789. doi: 10.1155/2023/9645789

Figure 1.

Figure 1

RRP reduces atherosclerosis-induced aging. (a) Representative Oil Red O staining of the entire aorta. (b) Representative images of H&E and Movat staining of aortic roots. (c) SA-β-gal staining to observe aortic vascular senescence in each group of mice. (d) Immunohistochemical assay to detect the levels of γ-H2AX in the blood vessels of each group of mice. (e) SASP levels in mice serum, including the levels of IL-1α, IL-1β, IL-6, MMP-3, TNF-α, TGF-β, and MCP-1. (f) Levels of senescence markers including NF-κB, p53, p21, p16, and γ-H2AX in total vascular proteins of mice were detected by western blot. Data are shown as mean values ± SD per group and expressed as fold-over the control mean. (g) The amounts of mRNA senescence markers NF-κB, p53, p21, and p16 in mouse vasculature were detected by qRT-PCR. Data are shown as mean values ± SD per group and expressed as fold-over the model mean (∗p < 0.05 and ∗∗p < 0.001 vs. control; #p < 0.05 and ##p < 0.001 vs. model by one-way ANOVA test).