Figure 7.

siAMPKα reversed the antisenescence effect of RRP. (a) Detection of γ-H2AX levels in HAECs after siAMPKα treatment by immunofluorescence assay. Scale bar = 50 μm. (b) Detection of senescence marker levels in HAECs after siAMPKα treatment by western blot. Data are shown as mean values ± SD per group and expressed as fold-over the control mean. (c) Representative staining images of SA-β-gal-positive cells in HAECs after siAMPKα treatment. Scale bar = 100 μm. (d) Detection of quantitative amounts of mRNA senescence markers in HAECs after siAMPKα treatment by qRT-PCR. Data are shown as mean values ± SD per group and expressed as fold-over the H₂O₂ mean (^∗p < 0.05 and ^∗∗p < 0.001 vs. control; ♢ = ns vs. siAMPKα+H₂O₂ by one-way ANOVA test).