Fig. 3.

Expression of downstream signaling pathway of STING. A The expression of P-TBK1/TBK1, P-IKK/IKK and P-NF-κB p65/NF-κB p65 in the L3-L5 DRGs of rats in the sham and BCP groups on day 14 were determined by western blotting. Representative protein bands were on the right. GAPDH was detected as an internal control. n = 8 per group. The expression and cellular localization of P-TBK1 (B), P-IKK (C) and P-NF-κB p65 (D) in the L3-L5 DRGs of rats in the sham and BCP groups on day 14 were determined by immunofluorescence. Representative IF images of P-TBK1 (red), P-IKK (red), P-NF-κB p65 (red), TuJ1 (green) and DAPI (blue) are shown on the right. The white arrows indicate typical co-stained cells. Scale bar = 50 μm. Mean fluorescence intensity (upper) and percentage of positive neurons in TuJ1⁺ DRG neurons (down) are shown on the left. Data are expressed as mean ± SEM. ****p < 0.0001. n = 4 per group (color figure online)