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. 2023 Feb 7;9:16. doi: 10.1038/s41421-022-00498-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunohistochemical verification of GCaMP expression in BF astrocytes. b The histogram shows that 93.5% of the GCaMP positive astrocytes were S100β positive. n = 8 brain slices. Scale, 40 µm. c–h Same as a and b, respectively, except that hM₃Dq, tdTomato, or ChrimsonR was expressed. The specificity for each group was 96.2%, 97.2%, or 100% and n = 8, 11, or 5 brain slices for each group, respectively.