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. 2023 Feb 6;13:2137. doi: 10.1038/s41598-023-28117-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Recovery and viability of GBS and monocytes from antibiotic protection assay developed for co-transcriptome analysis. Number estimates of viable GBS recovered from RPMI containing 1% FBS and other supplements, including antibiotics (250U/mL penicillin, 250U/mL streptomycin, and 50 µg/mL gentamicin) according to CFU assays were performed over a time-kill course post-addition of antibiotics (Abx) (A). Recovery of viable intracellular GBS (iGBS) from infected monocytes using protocols of no centrifugation as control (Ctrl), or centrifugation (500 × g, RT) for 5 min, or 50 min (B). The number (C) and viability (D) of monocytes recovered at 6 h post-infection, comparing Ctrl conditions without centrifugation and centrifugation for 5 min, or 50 min. Bars show means and SEMs representive of 4 independent experiments and analysed using One-way ANOVA with Holm Sidak Multiple comparisons. (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).