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. 2023 Feb 6;13:2137. doi: 10.1038/s41598-023-28117-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization of the interaction between human monocytes, WT GBS and Δsht GBS using fluorescence microscopy. The bacteria were tagged with either GFPmut3²⁴ or mCherry²² and were used to infect monocytes in single infection assays (one strain of GBS) or mixed infection assays (both strains of GBS tagged with a different fluorophore, used in equal numbers). The images were acquired using a Zeiss AxioImager.M2 microscope and Zen Pro (version 2) software.