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. 2023 Feb 6;14:638. doi: 10.1038/s41467-023-36298-2

Fig. 1. UBXD8 is enriched at ERMCS.

Fig. 1

a Immunoblot of the indicated proteins from subcellular fractionation of HEK293T cells. PNS post-nuclear supernatant, MAM mitochondria-associated membrane (n > 3 biologically independent samples). b Quantification plot showing the enrichment of UBXD8 in MAMs from a as normalized to calnexin. (n = 5 biologically independent samples). Data are means ± SEM (**P < 0.01, two-tailed paired t test). c Confocal microscopy showing enrichment of UBXD8 at ERMCS using Cos-7 cells transiently transfected with mito-BFP, SEC61β, and mCherry-UBXD8. Insets depict the zoomed in images. Scale bar, 10 μm. d Representative line-scan analyses of c showing the enrichment of mCherry-UBXD8 at ERMCS relative to SEC61β at ER as determined from n = 5 independent cells. Source data are provided as a Source data file.