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. 2023 Feb 6;14(2):86. doi: 10.1038/s41419-023-05631-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — CD4⁺IFN-γ⁺ Th1 cells, CD4⁺IL-4⁺ Th2 cells, CD4⁺IL-17A⁺ Th17 cells and CD4⁺Foxp3⁺ Treg cells in SP and PBMC from 6 and 14-week-old NOD mice were detected by flow cytometry. Representative flow cytometric analysis and percentages of CD4+ T cell subsets were shown. A Th1 cells in SP, B Th2 cells in SP, C the ratio of Th1/Th2 in SP, D Th1 cells in PBMC, E Th2 cells in PBMC, F the ratio of Th1/Th2 in PBMC, G Th17 cells in SP, H Treg cells in SP, I the ratio of Th17/Treg in SP, J Th17 cells in PBMC, K Treg cells in PBMC, L the ratio of Th17/Treg in PBMC. All the data are representative of two independent experiments. n = 5, *p < 0.05, **p < 0.01.