Fig. 7. Murine lung staining, scanning electron microscopy, and immunofluorescence show reduced levels of fibrotic-related lung damage following Bleo/TH5487 treatment compared to Bleo/vehicle samples.

a TH5487 significantly decreased lung damage in Bleo-treated mice (H&E) and b collagen deposition (picrosirius red) in both macroscopic and microscopic structures compared to vehicle/Bleo lungs and was confirmed by positive pixel analysis of whole-lung scanned images (scale bar of microscopic image =100 μm; scale bar of whole lung scan = 2 mm). Results shown from 3 independent experiments. Statistical analyses were conducted using a one-way ANOVA (*P < 0.05; **P < 0.01; ***P < 0.005). ns: not significant. Murine samples included in these analyses: Bleo n = 13, Bleo/TH n = 13, Bleo/Dex n = 8, Vehicle n = 8, TH only n = 9, Bleo/Pirf n = 7, Bleo/Nin n = 7). Bleo vs Bleo+TH displayed significantly reduced levels of H&E and picrosirius red staining (P < 0.0001). Data are presented as means ± SEM (a, b). c TH5487 /Bleo scanning electron microscopy images show reduced collagen deposition in the alveolar borders compared to Bleo-treated controls (scale bar = 20 μm). Immunofluorescent staining of murine lung sections (d) revealed decreased levels of MPO (red), fibronectin (red), OGG1 (red), and COL1A1 (green) following TH5487 treatment compared to both vehicle/Bleo and dexamethasone/Bleo groups (scale bar = 50 μm). e Co-stained murine lung sections revealed corresponding increases in OGG1 (red) and COL1A1 (green) following Bleo administration (DAPI counterstain, blue), with reduced levels of both OGG1 and COL1A1 in TH5487-treated samples (scale bar = 50 μm). Source data are provided as a Source data file.