TABLE 3.
Features of GA-1 ORFs
| ORF | No. of amino acids | Mol mass (kDa) | Putative translation initiation sitesa | Spacingb |
|---|---|---|---|---|
| B | 105 | 12.4 | ATACTTGGAAAGAAGGATGATAACA ATG | 11 |
| C | 94 | 10.7 | GAAACGCAAACTAGGAGGAAGAAAA ATG | 9 |
| D | 101 | 12.1 | TAATGGATAAAAGGGGGTAACAAAT ATG | 10 |
| E | 104 | 12.0 | ACGATAAAGCTTGGTTTGATAAAAA ATG | 14 |
| F | 25 | 2.7 | GAATGGTCTTAAAGGAGGTGATAAA ATG | 9 |
| G | 116 | 14.0 | CTCTAAATTATAAGGAGGAATTAAA ATG | 9 |
| H | 82 | 9.6 | ACATAACAAATAGGAGGAAACAAAA ATG | 10 |
| J | 80 | 9.5 | ACTCTAAGAAAGTTGGTAAATAAAA ATG | 11 |
| K | 48 | 5.8 | AAATTAAAGAATTGAGGTATATTGA ATG | 10 |
| L | 63 | 7.4 | GACATTAGAAAGGAGCTAGACAAAA ATG | 11 |
| M | 130 | 15.3 | GATAGTTAAAAGAGGAGGGTTATAA ATG | 9 |
| N | 33 | 3.5 | TATTAATTTAAAGGAGGAATTTTAA ATG | 10 |
| O | 94 | 11.1 | GTGTTATGATTAGAGGGGGATTCTA ATG | 8 |
| P | 56 | 6.6 | TAAAGATAGAAAGGGAGGGGATAAC ATG | 9 |
| Q | 141 | 16.4 | TTGATCATGTTAGGAGGAGAAAGTA ATG | 10 |
| R | 107 | 12.6 | AAGGGGAAACTAAAGGAGATGTCTA ATG | 8 |
| S | 29 | 3.6 | AAGGGGAAACTAAAGGAGATGTCTA ATG | 8 |
| T | 108 | 12.8 | AGATTCACTGAAAGGAGGTGATAAA ATG | 9 |
a
The putative initiation codons and the 25 nucleotides upstream are shown; nucleotides complementary to the 3′ end of B. subtilis 16S rRNA (UCUUUCCUCCACUAG) (152) are underlined.
b
The spacing is calculated as the distance (in bases) from the first base to the right of the 5′-AGAAAGGA-3′ (or the equivalent) to the base adjacent to the initiation codon.