Table 1.
Some universal primer combinations used for amplification of ribosomal RNA genes of PPNs.
| Primercombination and code(direction) | Primersequence(5′-3′) | Amplified region | References |
|---|---|---|---|
| G18SU(f) | GCTTGCCTCAAAGATTAAGCC | 18SrRNA | (Blaxter et al., 1998) |
| R18Tyl1(r) | GGTCCAAGAATTTCACCTCTC | (Chizhov et al., 2006) | |
| F18Tyl2(f) | CAGCCGCGGTAATTCCAGC | 18SrRNA | (Chizhov et al., 2006) |
| R18Tyl2(r) | CGGTGTGTACAAAGGGCAGG | ||
| 988F(f) | CTCAAAGATTAAGCCATGC | 18SrRNA | (Holterman et al., 2006) |
| 1912R(r | TTTACGGTCAGAACTAGGG | ||
| 1096F(f) | GGTAATTCTGGAGCTAATAC | 18SrRNA | (Holterman et al., 2006) |
| 1912R(r) | TTTACGGTCAGAACTAGGG | ||
| 1813F(f) | CTGCGTGAGAGGTGAAAT | 18SrRNA | (Holterman et al., 2006) |
| 2646R(r) | GCTACCTTGTTACGACTTTT | ||
| SSU_F_04 | GCTTGTCTCAAAGATTAAGCC | 18SrRNA | (Blaxter et al., 1998) |
| SSU_R_09 | AGCTGGAATTACCGCGGCTG | ||
| SSU_F_22 | TCCAAGGAAGGCAGCAGGC | 18SrRNA | (Blaxter et al., 1998) |
| SSU_R_13 | GGGCATCACAGACCTGTTA | ||
| SSU_F_23 | ATTCCGATAACGAGCGAGA | 18SrRNA | (Blaxter et al., 1998) |
| SSU_R_81 | TGATCCWKCYGCAGGTTCAC | ||
| designated Nem_18S_F |
CGCGAATRGCTCATTACAACAGC | 18SrRNA | (Floyd et al., 2005) |
| Nem_18S_R | GGGCGGTATCTGATCGCC | ||
| 18S-CL-F3 | CTTGTCTCAAAGATTAAGCCATGCAT | 18SrRNA+ ITS1-5.8S- ITS2rRNA+ 28SrRNA |
(Carta and Li, 2018; Carta and Li, 2019) |
| 28S-CL-R | CAGCTACTAGATGGTTCGATTAGTC | ||
| 18S(f) | TTGATTACGTCCCTGCCCTTT | ITS1-rRNA | (Vrain et al., 1992) |
| rDNA1.58S(r) | ACGAGCCGAGTGATCCACCG | (Szalanski et al., 1997) | |
| TW81(f) | GTTTCCGTAGGTGAACCTGC | ITS1-rRNA | (Curran et al., 1994) |
| 5.8SM5(r) | GGCGCAATGTGCATTCGA | (Zheng et al., 2000) | |
| 18S(f) | TTGATTACGTCCCTGCCCTTT | ITS1-5.8S- ITS2rRNA |
(Vrain et al., 1992) |
| 26S(r) | TTTCACTCGCCGTTACTAAGG | ||
| F194(f) | CGTAACAAGGTAGCTGTAG | ITS1-5.8S- ITS2rRNA |
(Ferris et al., 1993) |
| F195(r) | TCCTCCGCTAAATGATATG | ||
| TW81(f) | GTTTCCGTAGGTGAACCTGC | ITS1-5.8S- ITS2rRNA |
(Curran et al., 1994) |
| AB21(r) | ATATGCTTAAGTTCAGCGGGT | ||
| D2A(f) | ACAAGTACCGTGAGGGAAAGTTG | D2-D3of28S rRNA |
(Nunn, 1992) |
| D3B(r) | TCGGAAGGAACCAGCTACTA | ||
| D2Tyl(f) | GAGAGAGTTAAANAGBACGTGA | D2-D3of28S | (Chizhov et al., 2012) |
| D3B(r) | TCGGAAGGAACCAGCTACTA | rRNA | (Nunn, 1992) |
| D2A(f) | ACAAGTACCGTGAGGGAAAGTTG | D2of 28S rRNA |
(Nunn, 1992) |
| D2A(r) | GACCCGTCTTGAAACACGGA |