Table 3.
Information about reported studies of RPA in nematodes.
| Nematode species | Target | Time (min) | Temp (°C) | Sensitivity | References |
|---|---|---|---|---|---|
| M. enterolobii | IGS rRNA | 20 | 37 | 1/10 of a second-stage juvenile(J2) | (Subbotin, 2019) |
| M. javanica | SCAR marker | 40 | 39 | 1 pg purified genomic DNA, or 0.01 adult female, or 0.1 J2 | (Chi et al., 2020) |
| M. hapla | IGS rRNA | 20 | 39 | 1/100 of a J2 and 1/1000 of a female | (Subbotin and Burbridge, 2021) |
| M. enterolobii, M. incognita, M. javanica and M. arenaria | SCAR marker | 20 | 38 | 10−2, 10−2, 10−1, and 10−1 dilutions of DNA from a single J2 | (Ju et al., 2019) |
| H. schachtii | RAPD marker | 15-60 | 37 | 10−4 single cysts and single females, 4−3 single second-stage juveniles, and a 0.001 ng genomic DNA | (Yao et al., 2021) |
| H. avenae and H. filipjevi | SCAR marker | 15 | 35 | 10-4 single second-stage juvenile (J2), 10-5 single cyst, and 0.001 ng of genomic DNA | (Shao et al., 2022) |
| B. xylophilus | ITS2 | 25 | 37 | 308 ± 51 of B. xylophilus per 10 g of pinewood |