Figure 2.

Biosafety rAdV-ZW testing. Three-day-old piglets were orally administrated 2 mL 10¹⁰TCID₅₀ rAdV-ZW, 2 mL 10¹⁰TCID₅₀ rAdV-wild-type, and 2 mL PBS. (A) The rAdV-ZW effects on rectal temperature. After administration, piglet temperatures were measured daily. (B) Viral shedding determined by ELISA. After administration, feces samples were collected at one day intervals. (C) Piglet percentage weight gain after rAdV-ZW administration. Jejunal (D) IFN-γ and (E) IL-4 expression by ELISA at day 10 after oral administration. Values are presented as the mean ± standard error of the mean (SEM).