Skip to main content
. 2023 Jan 24;14:1058327. doi: 10.3389/fimmu.2023.1058327

Table 1.

Primers used to amplify four scFvs for recombinant adenovirus vector construction.

Primer Sequence (5’-3’) a
TrackZW1-16-BSalI TCGATGTCGACATGTACCCATACGATGTTCCAGATTACGCTGAGGAGAAGCTGGTGGAGTCCGG
TrackZW1-16-FHindIII TAACTAAGCTTTCAGAGCTCGTCCTTTTTGAGTTCCAGCTTGGTCCC
TrackZW3-21-BSalI TCGATGTCGACATGTACCCATACGATGTTCCAGATTACGCTGAGGTGAAGCTGGTGGAGTCTGG
TrackZW3-21-FHindIII TAACTAAGCTTTCAGAGCTCGTCCTTTTTGATCTCCAGCTTGGTCCC
TrackZW1-41-BSalI TCGATGTCGACATGTACCCATACGATGTTCCAGATTACGCTGAGGAGAAGCTGGTGGAGTCTGG
TrackZW1-41-FHindIII TAACTAAGCTTTCAGAGCTCGTCCTTTTTGAGCTCCAGCTTGGTCCC
TrackZW4-16-BSalI TCGATGTCGACATGTACCCATACGATGTTCCAGATTACGCTGAGGTGAAGCTGGTGGAGTCTGG
TrackZW4-16-FHindIII TAACTAAGCTTTCAGAGCTCGTCCTTGAGGACGGTCAGATGGGTCC
a

SalI and HindIII restriction sites are underlined.