Table 3.
Citrullination sites present in TSP-1, β-actin, and PF4 by PAD4 catalysis.
| protein | site | Annotated Sequence a | In vivo |
|---|---|---|---|
| TSP-1 (18) | R83 | FQDLVDAVRAEK | |
| R101 | TRGTLLALERK | ||
| R196 | DLASIARLRIAK | ||
| R216 | GGVNDNFQGVLQNVRFVFGTTPEDILR | ||
| R285 | DLQAICGISCDELSSMVLELRGLR | ||
| R288 | GLRTIVTTLQDSIR | ||
| R314 | RPPLCYHNGVQYR | R314 | |
| R404 | GRSCDSLNNR | ||
| R412 | SCDSLNNRCEGSSVQTR | R412 | |
| R421 | CEGSSVQTRTCHIQECDK | ||
| R458 | QDGGWSHWSPWSSCSVTCGDGVITRIR | ||
| R460 | IRLCNSPSPQMNGK | ||
| R517 | RSRLCNNPTPQFGGK | ||
| R607 | EVPDACFNHNGEHRCENTDPGYNCLPCPPR | ||
| R675 | CNYLGHYSDPMYRCECK | ||
| R751 | IPDDRDNCPFHYNPAQYDYDR | R751 | |
| R767 | DNCPFHYNPAQYDYDRDDVGDR | R767 | |
| R926 | DSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFR | R926 | |
| β-Actin (10) | R62 | RGILTLK | |
| R95 | IWHHTFYNELRVAPEEHPVLLTEAPLNPK | ||
| R183 | LDLAGRDLTDYLMK | ||
| R196 | ILTERGYSFTTTAER | ||
| R206 | GYSFTTTAEREIVR | ||
| R210 | REIVRDIK | ||
| R254 | SYELPDGQVITIGNERFR | ||
| R256 | FRCPEALFQPSFLGMESCGIHETTFNSIMK | R256 | |
| R312 | DLYANTVLSGGTTMYPGIADRMQK | ||
| R372 | QEYDESGPSIVHRK | ||
| PF4 (2) | R51 | TTSQVRPR | R51 |
| R80 | AGPHCPTAQLIATLKNGR*K | R80 |
a
red color indicates citrullinated arginine residue.