REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
LentiCas9-Blast	Addgene	Cat#52962
pDECKO_mCherry	Addgene	Cat#78534
Electrocompetent EnduraTM cells	Lucigen	Cat#60242-2
Chemicals, peptides, and recombinant proteins
Blasticidin	ThermoFisher	Cat#A1113903
Cytofix/Cytoperm Fixation	BD	Cat#51-2090KZ
DAPI	Roche	Cat#10236276001
Lipofectamine 2000	Thermofisher	Cat#11668019
Ki-67 Antibody	ThermoFisher	Cat#12-5698-82)
Matrigel® Matrix GFR, LDEV-free	Corning	Cat#356231
PE Annexin	ThermoFisher	Cat#L34960
Perm/Wash	BD	Cat#51-2091KE
Puromycin	ThermoFisher	Cat#A1113803
Trypan Blue Solution (100mL)	Sigma-Aldrich	Cat#T8154
Viability dye	ThermoFisher	Cat#35111
Critical commercial assays
Agencourt AMPure XP beads	Beckman Coulter	Cat#A63880
Blood & Cell Culture DNA Midi	Qiagen	Cat#13343
Blood & Cell Culture DNA Mini	Qiagen	Cat#13323
CellTrace™ CFSE Cell Proliferation Kit	ThermoFisher	Cat#C34570
CellTiter-Glo 2.0	Promega	Cat#G9242
CellTiter-Glo® 3D Cell Viability Assay	Promega	Cat#G9682
GoScript™ Reverse Transcriptase	Promega	Cat#A5003
GoTaq	Promega	Cat#M3001
GoTaq® qPCR Master Mix kit	Promega	Cat#A6002
QIAquick PCR Purification Kit	Qiagen	Cat#28104
Qubit™ dsDNA HS Assay Kit	ThermoFisher	Cat#Q32854
Quick-RNA™ kit from (#)	ZymoResearch	Cat#R1055
Z-Competent E.coli	ZymoResearch	Cat#T3001
Deposited data
Pooled CRISPR screens in A549 and NCI-H460	This paper	GEO: GSE207228
RNA-Seq of A549 and NCI-H460 upon ASO treatment	This paper	GEO: GSE207227
Experimental models: Cell lines
Human: A549 (male)	N/A	N/A
Human: CCD-16Lu (female)	N/A	N/A
Human: HEK293T (female)	N/A	N/A
Human: H441 (male)	N/A	N/A
Human: NCI-H460 (male)	N/A	N/A
Human: MRC-5 (male)	N/A	N/A
Experimental models: Organisms/strains
NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG® mice)	Charles River Laboratories	N/A
Oligonucleotides
LibDECKO-NSCLC1 (see File S1 for sequences)	This paper	N/A
ASOs (see Table S1 for sequences)	Qiagen	N/A
NGS sequencing primers (see Table S2 for sequences)	Sigma-Aldrich	N/A
Primers for genomic deletion and Primers RT-qPCR (see File S5)	Sigma-Aldrich	N/A
Software and algorithms
CRISPETa	92	N/A
FlowJo 10	FlowJo	N/A
Graphpad Prism	https://www.graphpad.com/scientific-software/prism/	N/A
R Statistical Software	https://www.r-project.org/	N/A
Target Prioritisation Pipeline (TPP)	https://doi.org/10.5281/zenodo.6912510	N/A
Stellaris probe designer	www.biosearchtech.com	N/A
Other
ChemiDoc Imaging System	BIO RAD	N/A
FACSAria™ III Cell Sorter	BD®	N/A
LSR II Flow Cytometer	BD®	N/A
Tecan Infinite® 200 Pro	N/A	N/A
Cell counter	Sigma-Aldrich	Cat#Z169021-1EA