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. 2022 Oct 19;102:1–51. doi: 10.3114/sim.2022.102.01

Table 3.

Support for delimitation of various species/populations using integrative approach consisting of four main components.

Examined species/populations Support from four evaluated components
Overall support (3–4/4)
GCPSR STACEY1 Single-locus MSC methods2 Morphology / physiology
A. campestris NO YES (2/2) YES (12/18) YES YES
segregation of A. campestris into three species NO3 NO (0/2) NO (6/18) NO NO
A. candidus YES YES (1/2) NO (7/18) N/A (trend) ?
A. dobrogensis YES YES (1/2) NO (7/18) N/A (trend) ?
Aspergillus sp. DTO 244-F1 N/A YES (2/2) YES (11/18) N/A ?
A. magnus N/A YES (2/2) YES (17/18) YES YES
A. neotritici YES YES (2/2) YES (18/18) YES YES
segregation of CCF 4914 and IBT 12659 from A. neotritici YES NO (0/2) NO (3/18) YES NO
A. pragensis YES YES (2/2) YES (17/18) YES YES
A. subalbidus YES YES (1/2) YES (17/18) YES YES
segregation of CCF 6199 and CCF 5642 (pop 6) from A. subalbidus YES YES (1/2) NO (9/18) NO NO
A. taichungensis YES YES (2/2) YES (13/18) YES YES
segregation of DTO 266-G2 from A. taichungensis NO NO (0/2) YES (10/18) NO NO
A. tenebricus YES YES (2/2) YES (13/18) YES YES

MCS – multispecies coalescent model-based methods; N/A – data not available or analysis could not be performed (non-viable strain DTO 244-F1 could not be analyzed phenotypically; species/populations represented by one strains could not be evaluated using GCPSR; a part of A. dobrogensis and A. candidus isolates cannot be distinguished morphologically – see sections Results, Discussion and Taxonomy).

1Support in at least one of the two most probable scenarios with collapseheight parameters 0.007 and 0.01 – see Fig. 4B.

2Support by the majority (at least 10 out of 18) of single-locus methods and their settings, i.e., agreement on the delimitation of species in its exact form or delimitation of smaller entities within it but without any admixture with related species/populations – see Fig. 3.

3Support is ambiguous: although three evolutionary lineages are supported by two out of three single-gene genealogies (Fig. 2), they lack support in the combined tree (Fig. 1); „YES“ scoring would not change the final decision about species limits using integrative approach.