FIG. 11.

Comparison of gene clusters involved in PA catabolism in different bacteria. Arrows show the directions of gene transcription. Genes are indicated by blocks: red (regulatory genes), dark blue (transport genes), light blue (genes encoding the PA-CoA ligase), green (genes encoding a putative multicomponent oxygenase), brown (genes encoding a putative ring cleavage enzyme), purple (genes encoding a putative β-oxidation-like pathway), and dotted (genes of unknown function). The paaI ortholog in P. putida U has not been described previously (182, 217), and it was named phaP here. orf3 is an incomplete orf gene that encodes a putative transcriptional regulator of the TetR family. An asterisk indicates a truncated gene. A discontinuous line means that the sequence has not been yet reported. The broken line shows that the genes are not adjacent. γ and β represent the γ and β subgroups of proteobacteria. The references of the sequences are as follows: E. coli (strain W) (accession no. X97452), K. pneumoniae (ERGO database), P. putida U and KT2440 (accession no. AF029714 and database referenced at website http: //www.ncbi.nlm.nih.gov/Microb_blast/unfinishedgenome.html, respectively), A. evansii KB740 (paa or pac genes under accession no. AF176259 or AJ278756, respectively), D. radiodurans R1 (accession no. AE002069), and B. halodurans C-125 (accession no. AB011837).