TABLE 2.
Ran binding proteins
| Name (mammalian) | Name (S. cerevisiae) | Affinity for Ran | GTP or GDP | Function | Reference(s) |
|---|---|---|---|---|---|
| Regulators | |||||
| RanGEF, RCC1 | Prp20 | 900 nM | Both | Guanine nucleotide exchange factor | 121, 122 |
| RanGAP | RNA1 | 430 nM | GTP | GTPase-activating protein | 120 |
| RanBP1 | Yrb1 | 1 nM | GTP | Coactivator of RanGAP | 132 |
| RanBP2 | 1 nM | GTP | Coactivator of RanGAP, binds SUMO-modified RanGAP | 277, 283 | |
| RanBP3 | Yrb2 | >5 μM | GTP | Cofactor for Crm1/Xpo1: activates RanGEF | 142, 256 |
| Nup50/NPAP60 | Nup2 | Low | GTP | Binds importinα and other karyopherins; cofactor? | 27, 85, 98, 240 |
| NTF2 | Ntf2 | 25 nM | GDP | Import carrier for Ran | 187, 206, 238 |
| NXT1, p15 | 12 nM | GTP | Cofactor for TAP (NXF1) and Crm1 | 23, 114, 252 | |
| G3BP1, G3BP2a, G3BP2b | Low | ? | ? | 114, 186 | |
| Mog1 | Mog1 | 200 nM | (GTP) | ? | 177, 244 |
| Cargo carriers | |||||
| Importinβ | Kap95 | 0/8 nM | GTP | Imports importinα; importinβ also imports XRIPα and snurportin adapters; ribosomal proteins, HIV Rev, HIV Tat, and HIV Rex proteins; Binds importin7, and RanBP8 | 40, 104, 105, 107, 182, 258, 263 |
| Transportin1, Kapβ2 | Kap104 | 1 nM | GTP | Imports mRNA binding proteins, ribosomal proteins | 5, 39, 105, 196 |
| Transportin SR | Mtr10 | 200 nM | GTP | Imports mRNA binding proteins (phospho-RS domains): Mtr10 imports Np13 | 115, 192, 233 |
| Hmtr10 | Mtr10 | ? | GTP | ? | 139, 192, 233 |
| Kap114 | ? | GTP | Imports TBP, histones H2A and H2B | 163, 165, 193 | |
| Yrb4, Kap123 | 2–3 nM | GTP | Imports ribosomal proteins | 227 | |
| Importin7 | 25 nM | GTP | Imports ribosomal proteins | 105 | |
| Importin11 | Lph2, Kap121 | >100 nM | GTP | Imports UbcM2 | 195 |
| Importin5, Kapβ3 | Pse1, Kap121 | ? | GTP | Imports ribosomal proteins, Pho4, and Spo12 | 49, 105, 281 |
| Nmd5, Kap119 | ? | GTP | Imports Hog1 and TFIIS | 6, 64 | |
| Sxm1, Kap108 | ? | GTP | Imports Lhp1 and ribosomal proteins | 213, 229 | |
| Pdr6, Kap122 | ? | GTP | TFIIA subunits | 257 | |
| RanBP16 | 200 nM | GTP | ? | 136 | |
| RanBP8, etc. | ? | GTP | ??? | 76 | |
| Exportin5 | Msn5, Kap142 | 40 nM | GTP | Kap142 exports phosphorylated proteins; imports RPA | 52, 110, 285 |
| Crm1 | Xpo1, exportin1 | 5–100 nM (cargo specific) | GTP | Export carrier for leucine-rich NES proteins, snurportin 1 | 69, 72, 129, 179, 241 |
| Cas | Cse1 | 1 nM | GTP | Exports importinα | 134, 135, 239 |
| Exportin t | Los1 | ? | GTP | Exports tRNAs | 8, 87, 138 |
| Exportin4 | 1.5–40 nM (± cargo) | GTP | Exports eIF5A | 144 |