Table 5.

Analyzed and calculated nutrient and amino acid (as-fed) of the basal diet with 45% of the estimated SID Met requirement (BASAL), reference diet (REF), and BSFL test diet with 55, 65, and 75% of the estimated SID Met requirement

Item	BASAL	REF55	REF65	REF75	BSFL55	BSFL65	BSFL75
Analyzed nutrient, %
Dry matter	91.89	92.20	92.29	92.27	91.60	92.04	90.88
Crude protein	11.59	11.70	12.30	12.71	13.57	12.68	12.12
Ash	3.60	3.55	3.88	3.70	4.02	3.80	4.14
Total indispensable AA, %
Arg	0.492	0.520	0.501	0.503	0.556	0.543	0.510
His	0.320	0.345	0.363	0.268	0.353	0.365	0.360
Ile	0.553	0.550	0.560	0.606	0.599	0.691	0.685
Leu	0.984	1.179	1.127	1.269	1.319	1.240	1.132
Lys	1.253	1.358	1.123	1.060	1.139	1.163	1.008
Met	0.141	0.188	0.194	0.212	0.200	0.212	0.240
Phe	0.672	0.723	0.681	0.720	0.745	0.714	0.681
Thr	0.696	1.504	0.442	0.195	0.325	0.809	0.695
Val	0.842	0.602	1.207	1.236	0.578	0.799	0.695
Total dispensable AA, %
Cys	0.169	0.172	0.152	0.152	0.183	0.180	0.191
Calculated crude protein,1 %	12.64	12.62	12.65	12.62	12.60	12.59	12.57
Calculated ME,2 kcal/kg	3,620	3,620	3,620	3,620	3,613	3,610	3,609
Calculated total indispensable AA,1 %
Arg	0.519	0.519	0.519	0.519	0.519	0.515	0.517
His	0.392	0.392	0.392	0.392	0.396	0.393	0.394
Ile	0.583	0.583	0.583	0.583	0.580	0.581	0.577
Leu	1.137	1.137	1.137	1.137	1.134	1.134	1.132
Lys	1.127	1.127	1.127	1.127	1.128	1.126	1.123
Met	0.131	0.160	0.190	0.220	0.160	0.189	0.218
Phe	0.673	0.673	0.673	0.673	0.675	0.674	0.673
Thr	0.673	0.673	0.673	0.673	0.673	0.672	0.674
Val	0.733	0.733	0.733	0.733	0.730	0.734	0.733
Calculated total dispensable AA,1 %
Cys	0.155	0.155	0.155	0.152	0.154	0.154	0.153

¹Calculated using an excel-based formulation software developed by Dr. Anna Kate Shoveller, using nutrient ingredient values from NRC, 2012 and PD-BSFL meal ingredient values from Enviroflight, LLC.

²ME, metabolizable energy. Calculated using the Atwater coefficients of 9 for crude fat, 4 for crude protein, and 4 for carbohydrate (NFE, calculated) (Atwater and Bryant, 1900).