Figure 3. Expression of NLRP3 in NSCLC cell lines results in increased ATM activation after the induction of DNA DSBs.

(A, B, C, D) A549 (A, B) or H292 (C, D) cells stably expressing a doxycycline-inducible NLRP3 lentiviral vector (pSLIK-NLRP3) induced (+Dox) or not (−Dox) with 0.5 µg/ml doxycycline for 24 h were irradiated with 2 Gy, and P-ATM (A, C) and γH2AX (B, D) foci were assessed 1 h post-treatment (n ≥ 103 cells). ****P < 0.0001, *P < 0.05 (unpaired t test).