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. 2023 Feb 6;6(4):e202201494. doi: 10.26508/lsa.202201494

Figure 4. NLRP3 controls the DDR in an inflammasome-independent manner.

Figure 4.

(A) HBEC3-KT control (siCTL) or caspase-1 siRNA (siCASP1) were treated with 100 µM of etoposide (Eto), and γH2AX phosphorylation was monitored by immunoblotting. Actin served as a loading control. (B) HBEC3-KT transfected with either control, NLRP3, or caspase-1 (CASP1) siRNA were treated with Eto (100 µM) 8 h, and IL-1β was quantified in cell supernatants by ELISA. Differentiated THP1 treated with nigericin for 3 h were used as a positive control for IL-1β secretion. (C) IL-1β secretion measurement at different time points in irradiated (2 Gy) HBEC3-KT transfected with control or NLRP3 siRNA using the Luminex assay. (D) HBEC3-KT cells were treated with 100 µM Eto over time, and caspase-1 cleavage was analyzed by immunoblotting. Actin served as a loading control. These data are one representative experiment out of two independent experiments. n.d, not detected.