Skip to main content
. 2023 Feb 6;6(4):e202201494. doi: 10.26508/lsa.202201494

Figure S3. NLRP3 is instrumental in achieving maximal ATM activation in response to DSBs in mammary epithelial cells.

Figure S3.

(A) HMEC-hTERT transfected with indicated siRNA were treated with 10 μM etoposide (Eto) and collected at different time points, and P-KAP1(Ser824) and P-CHK2 (Thr68) were analyzed by immunoblot. The ratio between P-KAP1/KAP1 and P-CHK2/CHK2 are shown. Representative of two independent experiments. (B) MDA-MB-231 cells transfected with indicated siRNA were treated with 0.5 μM Eto, fixed, and γH2AX foci were quantified by IF. Hoechst (blue) was used to stain nuclei (×40). Representative of three independent experiments. Unpaired t test P < 0.0001, Scale bar 20 μm. (C) MDA-MB-231 cells transfected with indicated siRNA were treated with 10 μM Eto and collected at different time points, P-KAP1 (ser824) and P-CHK2 (Thr68) were analyzed by immunoblot. The ratio between P-KAP1/KAP1 and P-CHK2/CHK2 are shown. Representative of two independent experiments. Actin was used as a loading control (A, C).