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. 2023 Feb 6;6(4):e202201494. doi: 10.26508/lsa.202201494

Figure S5. NLRP3 deficiency confers resistance to acute genotoxic stress but does not affect the extrinsic apoptosis pathway.

Figure S5.

(A) HBEC3-KT cells transfected with indicated siRNA in the absence or presence of an ATM inhibitor KU55933 (ATMi) were treated with etoposide (Eto) (50 μM) for 12 h and caspase-3/7 activity was assessed by luminometry. (B) Immunoblots show the efficacy of siRNA transfections. Actin served as a control. (C) HBEC3-KT cells transfected with control or NLRP3 siRNA were treated with TRAIL 200 ng/ml and MG132 1 mM for 12 h to induce death receptor-mediated apoptosis. Data represent mean ± SEM; ns, not significant (unpaired t test). Representative of two independent experiments. ****P < 0.0001, *P < 0.05 (unpaired t test).