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(A) Proximity ligation assay to detect the interaction between ATM/NLRP3 in HeLa cells transfected with an empty vector (EV) or FLAG-NLRP3. Scale bar 100 μm (×40). (B) Proximity ligation assay to detect the interaction between endogenous ATM/NLRP3 in MDA-MB-231 transfected with indicated siRNA and treated or not with 100 μM Eto for 1 h. ***P < 0.001, **P < 0.01 (unpaired t test). Scale bar 30 μm. Representative of two independent experiments.
