Figure 2.

Belated inhibitory effect of PI3K isoforms on NET formation. Neutrophils cultured at 37°C on poly-L-lysine-coated coverslips were exposed to 1 μM PI3Kα inhibitor IV (PI3Kα-selective) or 1 μM AS64002 (PI3Kγ-selective), either before (-15 min) or into (+60 or +120 min) a 4-h stimulation with 100 nM fMLF. NET formation was assessed using PlaNET Green as described in Methods. Mean ± s.e.m. of the standardized NET index from 3 independent experiments. *, p<0.05; **, p<0.01 vs stimulus alone. §, p < 0.001 for unstimulated cells vs stimulus.