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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Exp Neurol. 2023 Jan 7;361:114320. doi: 10.1016/j.expneurol.2023.114320

Fig. 3: The CP and pericontusional cortex are sites of acute endocannabinoid metabolism in the TBI mice.

Fig. 3:

Mouse brain sections were stained for cannabinoid enzymes by immunohistochemistry method. Endocannabinoid metabolizing enzymes FAAH, MAGL and Cox-2 showed higher expressions at 3 days and returned to reduced levels at day 26 post-injury (A-D). As seen at CP, the cortex also shows signs of acute metabolism of 2-AG and AEA as FAAH, MAGL and Cox-2 were highly elevated at day 3 post-TBI with respect to sham (E-H), while only cox-2 remained slightly elevated at day 26th (E, H). Groups were compared by Two-Way ANOVA analysis with Tukey’s post-hoc comparison. Results were calculated as mean fluorescent intensity (MFI) ± SEM. (n=5; ns= not significant; ***p<0.001; ****p<0.0001 vs. sham; Scale bar 125μm).