Figure 4.

Underrepresented PTM GSEA. GSEA were performed on the TARG1 tandem affinity purification of olaparib treated HEK293 cells published by Bütepage et al. Distributions of (A) p-values and (B) log₂ fold changes between treatment groups. (C) Volcano plot highlighting proteins with a log₂ fold change >1 and p < 0.05. (D) A Pearson’s Chi-squared test based on protein annotations (subcellular localization) indicates whether specific annotations are primarily found in significantly (p < 0.05) upregulated, downregulated, or nonsignificant (NS) proteins. Only localizations with positive residuals in the upregulated group are shown. The data in the figure are colored by Pearson residuals and sized by the absolute Pearson residuals. (E) GSEA using the urPTMdb sets highlighting the top 5 significantly enriched pathways (p_adj <0.05). (F) GSEA enrichment and volcano (G) plots for the urPTMdb set “PXD028902_hs_ADPribosylation_substrates”. Proteins found in the gene set are highlighted in the volcano plot.