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. 2023 Feb 5;61(1):391–403. doi: 10.1080/13880209.2023.2168019

Figure 6.

Figure 6.

FLL significantly stimulates osteoblast differentiation and inhibits adipocyte differentiation of BMMSCs. (A) The BMMSCs were cultured with FLL and subjected to CCK-8 assay (n = 6). (B,C) BMMSCs were cultured with the osteogenic-inducer solution for 7 d and we then determined ALP activity, and ALP was also stained with BCIP/NBT; FLL markedly promoted the expression of ALP. (n = 6). (D) The BMMSCs were cultured with the adipocyte-inducer solution for 7 d and stained with Oil Red. (E) We quantified Oil Red by dissolving it in isopropanol and measuring the absorbance at 500 nm. FLL significantly inhibited adipocyte differentiation (n = 6). (F) TOPFlash assay was used to investigate the activation of the Wnt-signaling pathway by FLL, and results showed that FLL significantly activated Wnt signaling (n = 6). (G,H) The levels of RUNX2 protein and mRNA in mouse bone marrow were noticeably increased after treatment with FLL (n = 3). (I,J) The levels of PPARγ protein and mRNA in mouse bone marrow were notably decreased after treatment with FLL. (n = 3). #p < 0.05 compared with sham or control; ##p < 0.01 compared with sham or control; *p < 0.05 compared OVX or model; **p < 0.01 compared OVX or Licl.