Table 2.

List of genomic DNA and genome-specific probes used for in situ hybridization

Name of probe	Description
gDNA_Ubriz1	Genomic DNA extracted from U. brizantha, CIAT 16341 (2n = 2x = 18)
gDNA_Udec1	Genomic DNA extracted from U. decumbens, CIAT 26305 (2n = 2x = 18)
gDNA_Udec2	Genomic DNA extracted from U. decumbens, CIAT 6133a (2n = 2x = 18)
gDNA_Uruz1	Genomic DNA extracted from U. ruziziensis, CIAT 26348 (2n = 2x = 18)
gDNA_Umax1	Genomic DNA extracted from U. maxima, CIAT 16049 (2n = 2x = 16)
gDNA_Umax2	Genomic DNA extracted from U. maxima, CIAT 6898 (2n = 2x = 16)
Uruz-spec1	Sequence found in U. ruziziensis, CIAT 26162 (2n = 2x = 18) by k-mer analysis
Udec2x-spec1	Sequence found in U. decumbens, CIAT 26305 (2n = 2x = 18) by RepeatExplorer
Udec2x-spec3	Sequence found in U. decumbens, CIAT 26305 (2n = 2x = 18) by RepeatExplorer
Udec2x-spec6	Sequence found in U. decumbens, CIAT 26305 (2n = 2x = 18) by RepeatExplorer
Udec4x-spec3	Sequence found in U. decumbens, CIAT 664 (2n = 4x = 36) by k-mer analysis
Ubriz-spec2	Sequence found in U. brizantha, CIAT 26032 (2n = 4x = 36), 26745 (2n = 4x = 36), 16292 (2n = 4x = 36) and 16290 (2n = 5x = 45) by k-mer analysis
Ubriz-spec3	Sequence found in U. brizantha, CIAT 26032 (2n = 4x = 36), 26745 (2n = 4x = 36), 16292 (2n = 4x = 36) and 16290 (2n = 5x = 45) by k-mer analysis
Uhum-spec1	Sequence found in U. humidicola, CIAT 26155 (2n = 6x = 36) by k-mer analysis
Uhum-spec3	Sequence found in U. humidicola, CIAT 26155 (2n = 6x = 36) by k-mer analysis
Uhum-spec7	Sequence found in U. humidicola, CIAT 26155 (2n = 6x = 36) by k-mer analysis
Uhum-spec12	Sequence found in U. humidicola, CIAT 26155 (2n = 6x = 36) by RepeatExplorer analysis

More details on genome-specific probes are given in Supplementary Data Tables S3 and S9. Results of in situ hybridization with selected probes are shown in Figs 5 and 6, and in Table 4. A summary of the research findings is presented in Fig. 7.