Figure 6. Eomes plays a critical role in maintenance of established SI CD8⁺ T_RM cells.

(A) Experimental design. CD8⁺ T cells from congenic control and Eomes^fl/flErt2-Cre⁺ (Eomes iKO) P14 mice were adoptively co-transferred at 1:1 ratio into recipients subsequently infected with LCMV. Mice received tamoxifen i.p. once daily x 5 doses starting at day 21 post-infection. Spleen and intestinal P14 T cells were harvested >10 days later.

(B and C) Representative flow cytometry plots (B) and quantification (C) of the proportions (top) or absolute numbers (bottom) of i.v.⁻ control vs. Eomes iKO P14 T cells in each intestinal tissue compartment.

(D) Representative flow cytometry plots (top) and bar graphs (bottom) indicating frequencies of control vs. Eomes iKO intestinal P14 T cells expressing CD69 and CD103.

(E) Proportions of control vs. Eomes iKO P14 T_RM cells among CD69⁺CD103⁺ (top) or CD69⁺CD103⁻ (bottom) subpopulations.

(F) Frequencies (top) or absolute numbers (bottom) of total control vs. Eomes iKO P14 T cells in the spleen.

(G) Bar graphs indicating the frequencies (top) or absolute numbers (bottom) of control vs. Eomes iKO T_CM (CD62L^hiCD127^hi), T_EM (CD62L^loCD127^hi), and t-T_EM (CD62L^loCD127^lo) cells.

Data are represented as mean ± SEM. Paired t-test. *p<0.05, **p<0.01, ***p<0.001 ****p<0.0001. Data are representative of ≥3 independent experiments with n=5–6 mice per experiment.

See also Figure S7.