Unique stem cell line identifier	NCHi009-A
Alternative name(s) of stem cell line	NCH78
Institution	Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH
Contact information of distributor	Mingtao Zhao, PhD
Type of cell line	iPSC
Origin	Human
Additional origin info required for human ESC or iPSC	Age: 20 years Sex: Female Ethnicity if known: Caucasian
Cell Source	Peripheral Blood Mononuclear Cells (PBMCs)
Clonality	Clonal
Method of reprogramming	Sendai virus vector expressing human KLF4, OCT3/4, SOX2, and c-MYC
Genetic Modification	Yes
Type of Genetic Modification	Spontaneous mutation
Evidence of the reprogramming transgene loss (including genomic copy if applicable)	RT-PCR for Kos transgene (Supplementary Fig. 1B)
Associated disease	Hypoplastic left heart syndrome (HLHS)
Gene/locus	NOTCH1: c.2058G > A (p.Gly661Ser) / chr9:136515323
Date archived/stock date	31st October 2022
Cell line repository/bank	NCHi009-A (NCH78) is deposited in the iPSC repository of pediatric cardiovascular disease in the Center for Cardiovascular Research at the Abigail Wexner Research Institute at Nationwide Children’s Hospital in Columbus, OH, USA. https://hpscreg.eu/cell-line/NCHi009-A
Ethical approval	Generation of this iPSC line was under an approved Institutional Review Board (IRB) protocol STUDY00001788 “iPSC Repository of Pediatric Cardiovascular Disease” at Nationwide Children’s Hospital.