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. Author manuscript; available in PMC: 2023 Feb 7.
Published in final edited form as: Methods Mol Biol. 2016;1401:121–134. doi: 10.1007/978-1-4939-3375-4_8

Fig. 1.

Fig. 1

Schematic for chromosomal engineering using lambda Red recombination and a sequential process of genetic transfer between K and B strains of E. coli. Table 2 primers are indicated in association with first and second PCR steps. PT7: T7 promoter; TT7: T7 terminator