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. Author manuscript; available in PMC: 2023 Feb 7.
Published in final edited form as: Methods Mol Biol. 2016;1401:121–134. doi: 10.1007/978-1-4939-3375-4_8

Table 2.

Primers used in the engineering of E. coli

Name Sequence
recA1 Forward: 5′-ATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCGTGTAGGCTGGAGCTGCTTC-3′
recA2 Reverse: 5′-CAGAACATATTGACTATCCGGTATTACCCGGCATGACAGGAGTAAAAATGATGGGAATTAGCCATGGTCC-3′
recA3 Forward: 5′-CTTGTCGAGCCCAAGGAACA-3′
recA4 Reverse: 5′-GAACCCGTCGTGGTGGAAAT-3′
pccAB1 Forward: 5′-GATCATATGGCTGCTCCGGCTTCTG-3′
pccAB2 Reverse: 5′-GTAGGATCCTTACAGCGGGATG-3′
cat1 Forward: 5′-GCGGAGCTCGTGTAGGCTGGAGCTGCTTC-3′
cat2 Reverse: 5′-GCGCTCGAGCATATGAATATCCTCCTTGG-3′
pccAB-cat1 Forward: 5′-TAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCAGATCTCGATCCCGCG-3′
pccAB-cat2 Reverse: 5′-ACCGGGCGTTAATAATTGTTTGTAAAGCGTTATTCGACACTGTTAGCCTCCAAAAAACCCCTCAAG-3′
pccAB-cat3 Forward: 5′-TCCTGCGTCGTTGGATCAGA-3′
pccAB-cat4 Reverse: 5′-GTGCCGGACTGATTCCAGTT-3′