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. 2023 Jan 31;2023:1367938. doi: 10.1155/2023/1367938

Figure 3.

Figure 3

CA9 confers resistance to ferroptosis in gefitinib-resistant cells. (a) Erastin sensitivity in parental (PC9 and HCC827) and gefitinib-resistant (PC9/GR and HCC827/GR) cells were analyzed by CCK8 assay to reflect ferroptosis sensitivity. CA9-knockdown resistant cells (b, c) and CA9-overexpressing parental cells (d) were treated with erastin; cell viability was measured by CCK8 assay. (e) Long-term colony formation of CA9-overexpressing parental cells in the treatment of erastin (10 μM). (f) Intracellular ROS were measured by flow cytometry (FITC channel) in CA9-overexpressing parental cells after erastin (10 μM) treatment for 48 h. (g, h) Intracellular labile iron were measured by immunofluorescence staining in CA9-overexpressing parental cells after erastin (10 μM) treatment for 48 h. The Mean ± SDs of three independent experiments are shown. (ns indicates not significant, ∗P < 0.05, ∗∗∗P < 0.001, Student's t-test).