Table 1.
The composition of the RT-qPCR reaction mixture.
| Components | Volume (μL) |
|---|---|
| 2 × mixture | 10 μL |
| PCR forward primer (10 μM) | 1 μL |
| PCR reverse primer (10 μM) | 1 μL |
| cDNA template | 1 μL |
| ddH2O | 7 μL |
| Total | Up to 20 μL |
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