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. 2023 Feb 7;14(2):87. doi: 10.1038/s41419-023-05625-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Experimental scheme. Trp53^fl/fl mice were crossed with Pvillin-Cre mice to generate Pvillin-Cre + Trp53^fl/fl (VP) mice, and the colorectal tumor model was induced by AOM/DSS. b Gene ontology analysis of gene sets that were significantly downregulated in metabolic process from VP mice CRC tissues compared to Trp53^fl/fl ones. c Gene set enrichment analysis (GSEA) was used to evaluate changes in the gene signature of peroxisomal FAO comparing CRC tumors from Trp53^fl/fl and VP mice. d Heatmap of RNA-seq expression values of peroxisomal FAO genes comparing tumors from VP and Trp53^fl/fl mice. e qRT-PCR analysis of various peroxisomal FAO genes comparing CRC tumors from Trp53^fl/fl and VP mice, n = 6. f Western blot analysis for ACOX1, EHHADH, ACAA1, SCP2, ACOX3, ABCD4, and p21 expression in CRC tumors from AOM/DSS-induced colitis-associated colorectal cancer (CAC) mouse model, n = 3. g HCT116 cells and MEFs were incubated with D43-C22:0 or D3-C22:0, whose catabolism to D39-C20:0 or D3-C16:0 was measured using mass spectrometry. Peroxisomal FAO was expressed as the ratio of D39-C20:0 to D43-C22:0 or D3-C16:0 to D3-C22:0; n = 3 or 4. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001.