. 2022 Oct 26;96(2):313–329. doi: 10.1007/s00420-022-01926-9

Table 1.

PCR primers and specific condition for A1AT, and APEX1 rs1130409 gene

Gene	Primers (reverse and forwards)	T (°C)	References
A1AT S & Z mutation	S (forward) 5′-TGA GGG GAA ACT ACA GCA CCT CG-3′, S (reverse) 5′-AGG TGT GGG CAG CTT CTT GGT CA-3′, Z (forward)5′-ATA AGG CTG TGC TGA CCA TCG TC-3′, Z(reverse) 5′-TTG GGT GGG ATT CAC CAC TTT TC-3′	Initial denaturation for 5 min. at 94 °C; 35 cycles of 1 min. at 94 °C, 1 min. at 55 °C, and 2 min. at 72 °C; final extension for 10 min. at 72 °C	Tazelaar et al. (1992)
APEX1 gene rs1130409 (Asp148Glu)	F 5′-CTG TTT CAT TTC TAT AGG CTA-3′, R 5′-AGG AAC TTG CGA AAG GCT TC-3′	Initial denaturation at 95 °C–5 min, 35 cycles of 95 °C–20 s, 55 °C–20 s, 72 °C–20 s and final extension at 72 °C–10 min	Lunn et al. (2000)

Gene

Primers (reverse and forwards)

T (°C)

References

A1AT S & Z mutation

S (forward) 5′-TGA GGG GAA ACT ACA GCA CCT CG-3′, S (reverse) 5′-AGG TGT GGG CAG CTT CTT GGT CA-3′, Z (forward)5′-ATA AGG CTG TGC TGA CCA TCG TC-3′,

Z(reverse) 5′-TTG GGT GGG ATT CAC CAC TTT TC-3′

Initial denaturation for 5 min. at 94 °C; 35 cycles of 1 min. at 94 °C, 1 min. at 55 °C, and 2 min. at 72 °C; final extension for 10 min. at 72 °C

Tazelaar et al. (1992)

APEX1 gene rs1130409 (Asp148Glu)

F 5′-CTG TTT CAT TTC TAT AGG CTA-3′, R 5′-AGG AAC TTG CGA AAG GCT TC-3′

Initial denaturation at 95 °C–5 min, 35 cycles of 95 °C–20 s, 55 °C–20 s, 72 °C–20 s and final extension at 72 °C–10 min

Lunn et al. (2000)