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. 2022 Oct 26;96(2):313–329. doi: 10.1007/s00420-022-01926-9

Table 2.

Methodology for restriction-RFLP product length

Gene Restriction enzyme RFLP products (bp)
A1AT Sand Z mutation Taq1 Fragments of (157 + 22 bp) and (100 + 21 bp) were detected for the wild type M allele, a 179 bp fragment for the Z allele and a 121 bp fragment for the S allele
APEX1 gene rs1130409 FspBI Asp/Asp: 164 bp; Asp/Glu: 164 bp, 144 bp, 20 bp; Glu/Glu: 144 bp, 20 bp